Product Description
FavorPrep GEL/PCR Extraction or Purification DNA fragments Mini Kit
This Purification Kit is designed to recover or concentrate DNA fragments (100bp-20Kb) from agarose gel, PCR or other enzymatic reaction. The unique dual purpose application and high yield DNA column make this kit exceptional value.
Specifications
Principle: spin column (silica matrix)
DNA Binding capacity of spin column: 20 µg
Sample size: up to 100 µl of reaction solution
DNA size: 65 bp ~ 10 kbp
Recovery: 85% ~ 95% for PCR clean-up
Operation time: ≤ 15 min
Elution volume: 40 µl
Important Notes:
1. Buffer provided in this kit contain irritants. Wear gloves and lab coat when handling these buffer.
2. Add the required volume of ethanol (96~100%) to Wash Buffer before use.
3. For concentraction or purification of DNA fragments from enzymatic reactions, the maximum sample volume is 100 µl and the maximum amount of DNA fragments is 5 µg.
4. Centrifugation steps are done by a microcentrifuge capable of the speed at 11,000 ~1,8000 x g.
Component | FAPCK 001(50 preps) | FAPCK 001-1(200 preps) | FAPCK 001-2(300 preps) |
FAPC Buffer | 30 ml | 110 ml | 180 ml |
Wash Buffer (concentrate) | 12.5 ml | 45 ml | 50 ml x 2 |
Elution Buffer | 5 ml | 20 ml | 20 ml |
FAPC Column | 50 pcs | 200 pcs | 300 pcs |
Collection Tube | 50 pcs | 200 pcs | 300 pcs |
Elution Tube | 50 pcs | 200 pcs | 300 pcs |
Storage:
Store at room temperature (15~ 25 ℃) for 2 year.