Product Description
Brain-Derived Neurotrophic Factor (BDNF) is a member of the neurotrophin family, essential for the development, survival, and plasticity of neurons in the central and peripheral nervous systems. It binds primarily to the TrkB receptor, triggering intracellular signaling cascades such as MAPK, PI3K, and PLCγ pathways, which regulate neuronal differentiation, synaptic growth, and long-term potentiation—key mechanisms underlying learning and memory.
BDNF is initially produced as a precursor protein (proBDNF), which is proteolytically cleaved to generate mature BDNF. Both forms have distinct biological functions: mature BDNF promotes survival and synaptic strengthening, while proBDNF can induce apoptosis or synaptic pruning through p75NTR signaling. BDNF expression is tightly regulated by neuronal activity and is influenced by environmental stimuli such as exercise and stress. Recombinant BDNF is commonly used in vitro and in vivo to study neural development, neurodegenerative diseases, psychiatric disorders, and to support the survival and growth of neurons in culture.
Applications
- Neuronal differentiation and outgrowth assays
- Neural stem cell maintenance
- Neuroprotection in in vitro or in vivo models
- Screening of neuroactive compounds
- Research in neuroscience applications such as depression, Alzheimer's disease, and TBI
Product Specifications
| Storage | -20°C |
| Storage Information | Avoid freeze/thaw cycles |
| Species | Human |
| Amino Acid Sequence | H S D P A R R G E L S V C D S I S E W V T A A D K K T A V D M S G G T V T V L E K V P V S K G Q L K Q Y F Y E T K C N P M G Y T K E G C R G I D K R H W N S Q C R T T Q S Y V R A L T M D S K K R I G W R F I R I D T S C V C T L T I K R G R |
| Molecular Weight | 13.4 kDa (Expected) |
| Class | Recombinant |
| Purity | > 95% by SDS-PAGE gel |
| Endotoxin Concentration | < 1 EU/µg |
| Biologically Active | Yes |
| Biological Activity | The activity of BDNF is determined by its ability to stimulate TrkB receptor phosphorylation and downstream signaling in relevant cell models. Each lot is tested to ensure consistent neurotrophic activity in culture. |
| Form | Lyophilized |
| Shipping Conditions | Ambient |
| Reconstitution | Reconstitute with sterile PBS + 0.1% BSA |
