Product Description
Accuris SmartCheck Tricolor Protein Markers are three-color protein standards with 10, 12, or 13 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer (3.5 kDa to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands, one green and one redband (at 25 kDa and 75 kDa, respectively, in the presence of
Tris-Glycine buffer).
Loading Volume
• 3 μl or 5 μl per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel.
• 1.5~2.5 μl per well for general Western blotting transfer.
The markers are supplied at aconcentration of approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Storage
This product is shipped at ambient temperature. Upon receipt, markers can be stored for up to 24 months at -20ºC or up to 3 months at 4ºC.
Quality Control
Under suggested conditions, SmartCheck™ Tricolor Protein Markers resolve 10, 12, or 13 major bands in SDS-PAGE (Tris-Glycine, MOPS, and MES buffer) and after Western blotting transfer to a nitrocellulose membrane.
General Guidelines
SmartCheck Protein Markers are supplied ready-touse. Gently pipette up and down to mix the markers and add up to 5µl to the desired well in the gel. If the markers are frozen, allow time to thaw and mix thoroughly.
