Product Description
PEC Chromogenic Agar is a selective medium specially formulated for the isolation and detection of E. coli, Pseudomonas aeruginosa and Candida albicans. These species are identifiable due to the mixture of chromogenic substrates and, additionally, tryptophan allows for Indole test to be performed for further E. coli confirmation.
PREPARATION
Mix 47.5 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. AVOID OVERHEATING. DO NOT AUTCLAVE.
QUALITY CONTROL SPECIFICATIONS
1. The powder is homogenous, free flowing and beige.
2. Visually the prepared medium is light to medium yellow, with trace to light haze.
3. Expected cultural response after 24-48 hours at 37 ºC.
ORGANISM | RESULT | |
Candida albicans ATCC 10231 | Good Growth – Green colonies | |
Enterococcus faecalis ATCC 19433 | Inhibited | |
Escherichia coli ATCC 25922 | Good Growth – Pink colonies / UV fluorescence | |
Staphylococcus aureus ATCC 25923 | Inhibited | |
Pseudomonas aeruginosa ATCC 27853 | Good Growth – Yellow colonies / UV fluorescence | |
Enterococcus faecalis ATCC 29212 | Inhibited | |
Staphylococcus aureus ATCC 6538 | Inhibited | |
Escherichia coli ATCC 8739 | Good Growth – Pink colonies / UV fluorescence | |
Pseudomonas aeruginosa ATCC 9027 | Good Growth – Yellow colonies / UV fluorescence |
STORAGE
Store the sealed bottle containing the dehydrated medium at 2 to 30°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original color.