Product Description
Vibrio Chromogenic Agar is recommended for the selective isolation and differentiation of Vibrio species based on colony colors, due to the enzymatic activities of ß-galactosidase and ß-glucosidase.
This medium is designed for the development and differentiation of 3 types of Vibrio depending on the enzyme activity of each strain. ß-glucosidase activity will appear as bluegreen colonies, as in the case of V. parahaemoliticus. The activity of ß-galactosidase enzyme will show red or pink colonies in the case of V. cholerae. And finally, the yellowish-white colonies will be V. alginolyticus, which has ß-galactosidase, but is not expressed due to the high concentration of sugars. The alkaline pH of the medium enhances the recovery of V. cholerae.
1. The powder is homogenous, free flowing and beige.
2. Visually the prepared medium is amber.
3. Expected cultural response after 24-48 hours at 35 ºC.
ORGANISM RESULT
Vibrio cholerae ATCC 14034 | Good Growth – Pink-rose colony | |
Vibrio alginolyticus ATCC 17749 | Good Growth – Colorless colony | |
Vibrio parahemolyticus ATCC 17802 | Good Growth – Green-blue colony | |
Escherichia coli ATCC 25922 | Inhibited | |
Vibrio vulnificus ATCC 27562 | Good Growth – Pink-rose colony |
STORAGE
Store the sealed bottle containing the dehydrated medium at 2 to 30°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original color.