Product Description
FavorPrep 96-well PCR Clean-Up Kit
Specifications:
Principle: Filter Plate (silica membrane)
Sample size: 10 ~ 100 µl of PCR mixture or other enzymatic reaction mixture.
DNA size: 65 bp ~ 10 kbp
Processing: centrifugation protocol or vacuum & centrifugation protocol
Operation time: < within 45 min/ 96 preparations
Recovery: 85% ~ 95% for PCR clean-up
DNA Binding capacity: up to 15 µg/ well
Elution volume: 50 ~ 75 µl
Downstream application: Fluorescent or radioactive sequencing, Restriction digestion, Library screening, Ligation, Labeling, Transformation.
Quality control
The quality of 96-Well PCR Clean-Up Kit is tested on a lot-to-lot basis. The purified DNA is checked by real-time PCR and capillary electrophoresis.
Important Notes:
1. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers. 2. Add ethanol (96~100%) Wash Buffer when first use. 3. Check FAPC Buffer before use, Warm Lysis Buffer at 60 °C for 5 minutes if any precipitate formd. 4. Components of this kit should be stored at 15 ~ 25 °C.
Component:
| FACKE 96001 | FACKE 96002 | FACKE 96004 | |
| FAPC Buffer | 60 ml | 120 ml | 120 ml x2 |
| Wash Buffer (concentrate) | 15 ml | 30 ml | 30 ml x2 |
| Elution Buffer | 15 ml | 30 ml | 30 ml x2 |
| Filter Plate (96-Well DNA Binding plate) | 1 plate | 2 plates | 4 plates |
| Collection Plate (96-Well 2 ml Plate) | 3 plate | 6 plates | 12 plates |
| Elution Plate (96-Well PCR plate) | 1 plate | 2 plates | 4 plates |
| Adhesive Film | 2 pcs | 4 pcs | 8 pcs |
Storage:
Components of this kit should be stored at 15 ~ 25 °C.
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