Product Description
FIREScript® KIT
Description
FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase.
This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C-60°C.
FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR).
Applications
- First-strand cDNA synthesis
- RT-PCR
- RT-qPCR
Properties
Sample type: RNA
Final product: cDNA (first strand)
Product format: Enzyme and reaction buffer (separate tubes)
Enzyme: FIREScript® Reverse Transcriptase
Source: Purified from an E.coli strain that carries an overproducing plasmid containing a FIREScript® Reverse Transcriptase gene.
Unit definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A)•oligo(dT) as a template in a total reaction volume of 50 μl.
Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Reagents
FIREScript® Reverse Transcriptase (200 U/μl)
10x RT Reaction Buffer with DTT (500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT)
Storage
Routine storage: -20ºC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
Shipping
At room temperature
Item |
Pcs. |
Vial size |
FIREScript® Reverse Transcriptase |
1 |
10000 U | 50 µl / 40000 U | 200 µl |
10x RT Reaction Buffer with DTT |
1 |
125 µl / 500 µl |