Product Description
FIREScript® RT cDNA synthesis KIT
Description
FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase.
This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C to 60°C.
FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR).
Applications
- First-strand cDNA synthesis
- RT-PCR
- RT-qPCR
Reagents
- FIREScript® Reverse Transcriptase (200 U/μl)
- RiboGrip® RNase inhibitor (220 U/μl)
- 10x RT Reaction Buffer with DTT (500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT)
- Oligo (dT) Primer (100 μM)
- Random Primers (100 μM)
- dNTP MIX (20 mM of each)
- Water, nuclease free
NB! A component of this kit has changed. RiboGrip® RNase Inhibitor (Cat. No. 06-22) has been discontinued and is replaced by a new product: RiboGrip® RNase Inhibitor 220 U/µl. Please refer to the product data sheet for up-to-date usage instructions.
Storage
Routine storage: -20 ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
Shipping
At room temperature.
|
Item |
Pcs. |
Vial size |
|
FIREScript® Reverse Transcriptase |
1 |
10000 U | 50 µl / 40000 U | 200 µl |
|
RiboGrip® RNase Inhibitor (220 U/µl) |
1 |
2000 U | 9 μl / 4000 U | 18 μl |
|
10x RT Reaction Buffer with DTT |
1 |
125 µl / 500 µl |
|
dNTP MIX (20 mM of each) |
1 |
2 µmol | 25 µl / 8 µmol |
|
Oligo (dT) Primer |
1 |
50 µl / 200 µl |
|
Random Primers |
1 |
50 µl / 200 µl |
|
Water, nuclease free |
1 / 3 |
1.25 ml |
