FIREScript® RT cDNA synthesis MIX with Random primers

Product Description

FIREScript® RT cDNA synthesis MIX with Random primers

Description

FIREScript^® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase.

This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C to 60°C.

FIREScript^® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR).

Applications

• First-strand cDNA synthesis
• RT-PCR
• RT-qPCR

Properties

Sample type: RNA

Final product: cDNA (first strand)

Product format: Enzyme mix with all reagents included

Enzyme: FIREScript^® Reverse Transcriptase

Source: Purified from an E.coli strain that carries an overproducing plasmid containing a FIREScript^® Reverse Transcriptase gene.

Unit description: One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(rA)•oligo(dT) as template in a total reaction volume of 50 μl.

Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Reagents

FIREScript^® Enzyme Mix (FIREScript^® RT and RiboGrip^® RNase inhibitor)

10x RT Reaction Premix with Random primers
RT reaction buffer with DTT, dNTPs and Random primers

Water, nuclease free

Storage

Routine storage: -20ºC

Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.

Shipping

At room temperature.