Product Description
HOT FIREPol® GC Master Mix
Description
HOT FIREPol® GC Master Mix has been developed for working with difficult GC-rich templates and DNA secondary structures. It shows excellent amplification with templates up to 79% GC content
The master mix contains hot-start Taq polymerase HOT FIREPol®, MgCl2, dNTPs, and a special buffer for high yield GC-rich amplification.
Extra vials of 100% DMSO and 25 mM MgCl2 enable flexibility in reaction optimization.
Properties
Concentration: 5x
Hot-start: yes, initial activation in 12-15 min.
Ready to load: no
Fidelity: 1 x Taq
Cloning Type: T/A cloning
Amplicon Size: up to 5 kb
Difficult templates: robust on GC-rich templates
Mix Composition:
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
5x HOT FIREPol® GC Buffer with 7.5 mM MgCl2: 1x PCR solution –1.5 mM MgCl2
dNTPs: dATP, dCTP, dGTP and dTTP
Bovine serum albumin (BSA) to enhance PCR reaction
In separate vials:
100% DMSO is included in the kit in a separate vial. DMSO is recommended as a PCR additive for templates with high GC content. In some cases, DMSO is also required to relax secondary structures.
25 mM MgCl2 for further optimization
Storage
Routine storage: -20ºC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of 5x HOT FIREPol® GC Master Mix
Shipping
At room temperature
|
Item |
Pcs. |
Vial size |
|
HOT FIREPol® GC Master Mix |
1 |
1 ml | 250 rxn |
|
DMSO 100% |
1 |
0.5 ml |
|
25 mM MgCl2 |
1 |
0.5 ml |
