HOT FIREPol® MultiPlex Mix

Product Description

HOT FIREPol® MultiPlex

Description

HOT FIREPol^® MultiPlex Mix is a premixed ready-to-use solution containing all reagents required for hot-start multiplex PCR (except template, primers and water).

Generated PCR products are compatible with TA cloning procedures

Properties

Concentration: 5x

Hot-start: yes, initial activation in 12-15 min

Ready to load: no

Fidelity: 1 x Taq

Cloning Type: T/A cloning 

Multiplexing: up to 18 targets (depending on amplicon length)

Amplicon Size: up to 5 kb

Mix Components

• HOT FIREPol^® DNA polymerase
• 5x Multiplex Buffer with 10 mM MgCl₂ (1x PCR solution – 2 mM MgCl₂)
• dNTPs 
• BSA
• Compound that increases sample density (loading dyes not included)

Storage

Routine storage: -20ºC

Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol^® Multiplex Mix

Shipping

At room temperature