Product Description
HOT FIREPol® Multiplex qPCR Mix (ROX)
Description
HOT FIREPol® Multiplex qPCR Mix (ROX) is optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays. The qPCR Mix comprises all the components necessary (except primers, probes and template) to perform qPCR: HOT FIREPol® DNA Polymerase, optimized buffer components, ultrapure dNTPs, MgCl2 and Purple passive reference dye according to system requirements.
HOT FIREPol® Multiplex qPCR Mix (ROX) is optimized for DNA hydrolysis probes based on the 5' flap endonuclease activity.
HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Applications
- detection and quantification of DNA and cDNA targets
- profiling gene expression
- microbial detection
- viral load determination
Properties
Concentration: 5x
Hot-start: yes, initial activation in 10 min
Detection type: Probe-based
Reference dye: ROX
Suitable qPCR Cyclers: qPCR platforms from Applied Biosystems and Agilent/Stratagene.
Mix Components
HOT FIREPol® DNA Polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start
5x Multiplex qPCR buffer with 15 mM MgCl2: 1x PCR solution – 3mM MgCl2
dNTPs including dUTP: the mix allows UNG treatment to prevent carryover contamination from previous runs.
IMPORTANT: UNG is not included in the HOT FIREPol® Multiplex qPCR Mix
ROX dye: If ROX, Texas Red, or similar dye is used as one of the fluorophores, ROX passive reference dye might interfere with the signal. A version without passive reference or with Purple reference dye is available.
Storage
Routine storage: -20°C
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® Multiplex qPCR Mix.
Shipping
At room temperature
