Product Description
HOT FIREPol® SolisGreen qPCR Mix 2.0
Description
HOT FIREPol® SolisGreen® qPCR Mix 2.0 is a 5x-concentrated solution specially designed for dye-based real-time quantitative PCR (qPCR) assays. SolisGreen® dsDNA binding dye is incorporated into the product to evaluate the DNA amplification process during PCR. The fluorescence spectra of this dye are similar to the more widely used SYBR® Green I and is compatible with most major real-time cyclers. When compared to SYBR® Green I, SolisGreen® dye shows:
- higher fluorescence level
- better sensitivity for detecting low template concentrations
- great stability for room temperature storage
HOT FIREPol® SolisGreen® qPCR Mix 2.0 is an optimized ready-to-use solution that contains all the necessary components, except sample (DNA template) and primers, to perform reactions with accurate and highly sensitive results. Additionally, the product includes a passive reference based on ROX dye making it compatible with both ROX-dependent and ROX-independent qPCR cyclers.
The product contains HOT FIREPol® DNA Polymerase to prevent the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Applications
- Gene expression analysis and absolute quantification
- Pathogen detection and quantification
- Low-copy gene detection
- Cell-free DNA analysis
Properties
Sample type: DNA
Concentration: 5x
Hot-start: Yes, initial activation in 10 min
Detection type: Dye-based, includes SolisGreen® intercalating dye
Reference dye: Based on ROX
Compatible real-time instruments: The mix is compatible with both ROX-dependent and ROX-independent qPCR cyclers. HOT FIREPol® SolisGreen qPCR Mix 2.0 is not compatible with platforms that require high ROX, such as Applied BioSystems® 7900HT, StepOne™ or StepOnePlus™ systems.
Mix Components
HOT FIREPol® DNA polymerase: chemically modified FIREPol® DNA Polymerase enabling hot-start.
5x qPCR buffer with 12.5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
dNTPs: dATP, dCTP, dGTP and dTTP
SolisGreen® dye
Internal reference based on ROX dye
Storage
Routine storage: -20ºC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® SolisGreen® qPCR Mix 2.0.
Shipping
At room temperature.
