Product Description
HOT TERMIPol® DNA Polymerase
Description
HOT TERMIPol® DNA polymerase is a chemically modified TERMIPol® DNA polymerase. At ambient temperature it is inactive, having no polymerase activity.
HOT TERMIPol® DNA polymerase is fully activated by a 12-15 minute incubation at 95ºC. This prevents the extension of non-specifically annealed primers. The enzyme contains 5’→3’ polymerase activity and enhanced efficiency for incorporating unconventional nucleotides (ddNTPs and labeled ddNTPs).
Applications
- MALDI-TOF mass spectrometry
- Primer extension
- Mass-array
Properties
Concentration: 5 U/µl
Hot-start: yes, activated by 12-15 minutes at 95ºC
Error rate per nucleotide per cycle is app. 8 x 10-5
Estimated half life at 95ºC is 1.5 hours.
Storage and Dilution Buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.
Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 74ºC.
Reagents
HOT TERMIPol® DNA Polymerase
10x Reaction buffer C: 500 mM Tris-HCl pH 9.5 at 25ºC
100 mM MgCl2
Storage
Routine storage: -20ºC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT TERMIPol® DNA polymerase.
Shipping
At room temperature
|
Item |
Pcs. |
Vial size |
|
HOT TERMIPol® DNA Polymerase |
1 |
500 U | 100 µl |
|
10x Reaction Buffer C |
1 |
5 ml |
|
100 mM MgCl2 |
1 |
5 ml |
