Product Description
- Used in agarose electrophoresis to follow the migration of nucleic acids
The 6X Gel Electrophoresis Loading Dye is ideally suited for agarose electrophoresis of DNA, RNA, or nucleic acids. Its ultra-pure grade ensures quality and repeatability with every run. The light blue, indigo, and magenta dyes will clearly show the migration of each run and the dyes migrate independently from the samples allowing the estimation of nucleic acids.
The 6X Gel Loading Dye contains 15% Ficoll in a special Tris dye. The 6X Loading Dye contains three tracking dyes that make estimating sample migration easy and reliable.
Tracking Dye #1 is light blue and migrates at around 4 Kb in a 1% agarose gel (similar to Xylene Cyanol).
Tracking Dye #2 is indigo and migrates at around 600 bp in a 1% agarose gel (similar to Bromophenol Blue).
Tracking Dye #3 is magenta and migrates at around 150 base pairs in a 1% agarose gel.
CAS#N/A
Abs. @ 525 nm (1:500 water)0.3-0.6
Abs. @ 588-594 nm (1:500 water)0.5-0.8
Abs. @ 635-641 nm (1:500 water)0.5-0.7
Recommended Use
The 6X Loading Dye contains 15% Ficoll in a special TRIS Dye. It is ideal for DNA or RNA gels.
The 6X Loading Dye contains three tracking dyes that make estimating sample migration easy and reliable.
Dye #1: Light blue slightly slower than Xylene Cyanol migrating at around 4 base pairs in a 1% agarose gel.
Dye #2: Indigo dye migrates similar to Bromophenol Blue at around 600 base pairs in a 1% agarose gel.
Dye #3: Magenta dye migrates at around 150 base pairs in a 1% agarose gel.
The dyes migrate independently from the samples, allowing the estimation of nucleic acids. The loading dyes impart color to the samples, which visually facilitates the loading process. The loading dyes increase the density of the samples ensuring even loading of the sample wells.
What is the “Special TRIS” buffer that makes up the 6X loading dye?
The buffer no longer contains a TRIS buffer. The buffer now uses DI water to lower the concentration from 25X to 6X.
