Product Description
Milenia Biotec HybriDetect Chase Buffer B (MHCBB 1)
Optimized Buffer for RPA and LAMP
The HybriDetect Chase Buffer B (MHCBB 1) is a specialized running buffer explicitly formulated to optimize the performance of Milenia HybriDetect lateral flow strips when analyzing amplicons from Isothermal Amplification methods, specifically RPA (Recombinase Polymerase Amplification) and LAMP (Loop-mediated Isothermal Amplification). Unlike the standard TRIS-based buffers, this solution uses a Phosphate/Citrate base with a lower pH (6.0) and includes specific blocking reagents to reduce non-specific binding often found in these highly sensitive reactions.

Specialized for Isothermal Methods
While the standard buffer (MHCB 1) covers general needs and Buffer A (MHCBA 1) handles PCR, Buffer B addresses the unique chemical conditions of isothermal assays. It is the recommended choice for researchers developing RPA or LAMP assays on the HybriDetect platform (MGHD 1, MGHD 2, MGHC 1) who require cleaner backgrounds and sharper signal definition.
Key Features
- Isothermal Optimization: Specifically designed for the evaluation of RPA and LAMP amplicons.
- Unique Formulation: Phosphate/Citrate-buffered saline at pH 6.0 with blocking reagents.
- Universal Compatibility: Works with all HybriDetect dipsticks and cassettes (MGHD 1, MGHD 2, MGHC 1).
- Ready-to-Use: Supplied in a 10 mL dropper bottle for immediate application.
Technical Specifications
| Parameter | Specification |
|---|---|
| Catalog Number | MHCBB 1 |
| Volume | 10 mL |
| Composition | Phosphate/Citrate-buffered saline, pH 6.0 |
| Additives | 0.05% Sodium Azide, Blocking reagents |
| Primary Application | Detection of RPA and LAMP amplicons |
| Storage | 2°C to 8°C |