MLP - Multiplex Gastrointestinal Assay, 96 well, 6 panels per plate (Breakaway Plates)

Product Description

The Gastrointestinal (GI) Pathogen Panel is a multiplex polymerase chain reaction (PCR) assay for the identification of nucleic acids from 16 organisms frequently associated with GI tract infections. This method is highly accurate and analytically sensitive It is used to identify organisms by amplifying and detecting genetic material of pathogens in samples. The panel will aid in the research of causative agents of GI tract infections and their prevalence.

The target organisms included in the panel are as follows:
C. difficile: C. difficile tcdA, C. difficile tcdB
E. coli and Shigella spp.: Enterotoxigenic E. coli (ETEC), Shiga-like toxin producing E. coli (STEC), Shigella/Entroinvasive E. coli (EIEC)
Parasites: Cryptosporidium spp., Entamoeba histolytica, and Giardia lamblia
Other GI Bacteria: Campylobacter spp., Salmonella spp., Yersinia enterocolitica, Vibrio spp.
Viruses: Adenovirus F40/F41, Norovirus GI/GII, Rotavirus A, Astrovirus

Usage Information

	Reagent Storage and Use Guidelines
	Store all reagents at -25°C to -15°C Do not freeze-thaw plates more than 3 times.
	The Following is Included in the Kit
	96 well PCR plate pre-loaded with the Gastrointestinal Pathogen Panel assays and positive and negative controls
	The Following is Supplied by the User: Materials
	Extracted Sample(s) qPCR optical film Sealer for optical film
	Equipment
	Manual defrost -20°C freezer Laminar Flow or PCR Dead Air Box for general plate setup. Do not use Laminar Flow for infectious samples Pipettes and appropriate filtered pipette tips Plate Vortex [recommend Vortex Genie 2 (Model G560) with the 3-inch platform and rubber cover] Plate centrifuge Lab utility knife
	Instrumentation
	CFX96 Touch Real-Time PCR Detection System (or equivalent)

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Limitations of Use
For Research Use Only. Not for use in diagnostic procedures.