MLP - MultiPlex Urinary Tract Infection Assay including ABX Resistance, 384 well, 12 panels per plate

Product Description

The Urinary Tract Panel Plus is an in-vitro multiplex real-time polymerase chain reaction (RT-PCR) assay for the qualitative identification of nucleic acids from organisms frequently found in the urinary tract. This method is highly accurate, analytically sensitive and is used to identify organisms by amplifying and detecting genetic material of pathogens in samples. The panel will aid in the research of causative agents of urinary tract infections and their prevalence.

The target organisms included in the panel are as follows: Acinetobacter baumannii, Bacteroides fragilis, Candida albicans, Candida dubliniensis, Candida glabrata, Candida krusei, Candida parapsilosis, Candida tropicalis, Citrobacter braakii/ freundii, Citrobacter koseri, Enterobacter cloacae, Enterococcus spp., Escherichia coli, Klebsiella aerogenes, Klebsiella oxytoca / michiganensis, Klebsiella pneumoniae, Morganella morganii, Mycoplasma genitalium, Mycoplasma hominis, Prevotella bivia, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Staphylococcus epidemidis, Staphylococcus saprophyticus, Streptococcus agalactiae (Group B), Streptococcus pyogenes (Group A), and Ureaplasma urealyticum.

Antibiotic Resistance Markers present are as follows: β-lactamase (blaKPC), β-lactamase (CTX-M-Group 1), metallo-βlactamase (blaNDM), Fluoroquinolones, Methicillin/Oxacillin (mecA), Sulfonamides, Trimethoprim, and Vancomycin (vanA, van B)

Usage Information

	Reagent Storage and Use Guidelines
	Store all reagents at -25°C to -15°C Do not freeze-thaw plates more than 3 times.
	The Following is Included in the Kit
	384-well PCR plate pre-loaded with the Urinary Tract Plus Microbiota assay mix and positive and negative controls
	The Following is Supplied by the User: Materials
	1. Extracted Sample(s) 2. PCR optical film 3. Sealer for optical film
	Equipment
	Manual defrost -20°C freezer Laminar Flow or PCR Dead Air Box for general plate setup. Do not use Laminar Flow for infectious samples Pipettes and appropriate filtered pipette tips Plate Vortex Plate centrifuge Lab utility knife
	Instrumentation
	CFX384 Touch Real-Time PCR Detection System

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Limitations of Use

For Research Use Only. Not for use in diagnostic procedures.

Disclaimer - Use of PCR and Patent

This product is for basic PCR and is outside of any valid US patents assigned to Hoffman La-Roche.