Product Description
Green Fluorescent Protein (GFP) is a naturally occurring protein from the jellyfish Aequorea victoria that emits bright green light during bioluminescence. Widely used as a reporter and protein tag in both eukaryotic and prokaryotic systems, GFP enables direct visualization of gene expression, protein localization, and dynamic cellular processes in living cells and organisms.
As a 27 kDa monomeric protein, GFP autocatalytically forms its fluorescent chromophore without the need for additional proteins, substrates, or cofactors. The wild-type protein absorbs blue light (peak at ~395 nm) and emits green light (peak at ~508 nm), with fluorescence that is highly stable, species-independent, and suitable for a variety of imaging applications.
GFP fusion tagging allows researchers to:
- Track protein localization and trafficking in live cells.
- Study subcellular compartment dynamics.
- Monitor gene expression patterns.
- Investigate protein–protein interactions (e.g., yeast two-hybrid system).
- Measure molecular proximity via Förster Resonance Energy Transfer (FRET) assays.
This monoclonal antibody specifically recognizes the GFP epitope, providing sensitive and specific detection in applications such as Western blotting, immunoprecipitation, immunofluorescence, and ELISA. It is suitable for detecting both native and denatured GFP and its common variants, ensuring consistent performance in research workflows.
