Product Description
Goat Anti-Mouse IgG (H&L) HRP is an affinity-purified secondary antibody with well-defined specificity for mouse immunoglobulins. It binds to both heavy (H) and light (L) chains, ensuring broad recognition of mouse IgG subclasses.
Secondary antibodies provide increased flexibility and sensitivity in detection systems by amplifying signal output—multiple secondary antibodies can bind to a single primary antibody. Conjugation to horseradish peroxidase (HRP) enables reliable use in enzyme-based detection methods such as chemiluminescence and colorimetric assays.
This antibody is generated by immunizing goats with purified mouse immunoglobulins and is further purified to ensure high specificity and low background. The HRP conjugation allows sensitive, consistent, and reproducible performance in applications such as Western blotting.
Applications
- ELISA: 1:5000 – 1:20000
- WB: 1:2000 – 1:20000
- IHC: 1:500 – 1:1000
- ICC: 1:500 – 1:1000
Product Specifications
| Shipping | Dry Ice |
| Storage |
-20°C |
| Storage Information | Avoid freeze/thaw cycles |
| Species | Goat |
| Purity | The antibody was purified by immunoaffinity chromatography using antigens coupled to agarose beads. |
| Form | Liquid |
| Isotype | IgG |
| Description | Goat Anti-Mouse IgG (H&L) conjugated to HRP. |
| Concentration | 1 mg/mL |
| Clonality | Polyclonal |
| Specificity | By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG. No antibody was detected against non immunoglobulin serum proteins. |
| Immunogen | Mouse IgG. |
| Buffer | Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.05% Sodium Azide. |
| Reactivity | Mouse |
