Product Description
Glutathione S-transferase (GST) is a 26 kDa protein found in both eukaryotic and prokaryotic organisms, where it catalyzes a variety of biochemical reactions. In research applications, the GST gene is frequently used in fusion expression systems, producing GST-tagged proteins that can be efficiently purified via their strong affinity for glutathione.
GST fusion proteins are widely utilized in protein research, particularly for studying direct protein–protein interactions. The GST tag system is compatible with numerous expression vectors, making it a versatile and widely adopted tool for recombinant protein production and purification.
This monoclonal antibody specifically recognizes the GST epitope, enabling sensitive and specific detection of GST-tagged proteins in applications such as Western blotting, immunoprecipitation, immunofluorescence, and ELISA. It provides reliable performance for both native and denatured forms, ensuring consistent results across diverse experimental workflows.
Applications
- WB: 1:2000 – 1:5000
- IP: 1:100 – 1:200
Product Specifications
| Shipping | Dry Ice |
| Storage |
Short Term: 1 month, 4°C; Long Term: 12 months, -20°C |
| Storage Information | Avoid freeze/thaw cycles |
| Species | Mouse |
| Purity | The antibody was purified by immunogen affinity chromatography. |
| Form | Liquid |
| Isotype | IgG |
| Description | Mouse monoclonal antibody to GST-tag |
| Concentration | 1 mg/mL |
| Clonality | Monoclonal |
| Specificity | Recognizes C-terminal, internal, and N-terminal GST-tag fusion proteins. |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence of GST-tag. The exact sequence is proprietary. |
| Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
