Product Description
Rabbit Anti-Human IgG (H&L) HRP is an affinity-purified secondary antibody with well-defined specificity for human immunoglobulins. It binds to both heavy (H) and light (L) chains, ensuring broad detection of human IgG subclasses.
Secondary antibodies increase assay sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. Conjugation to horseradish peroxidase (HRP) enables robust detection in enzyme-based systems such as chemiluminescence and colorimetric assays.
This antibody is produced by immunizing rabbits with purified human immunoglobulins and subsequently affinity-purified to ensure high specificity and low background reactivity. The HRP conjugation ensures sensitive, consistent, and reproducible performance in multiple applications including Western blotting.
Applications
- ELISA: 1:5000 – 1:20000
- WB: 1:5000 – 1:20000
- IHC: 1:500 – 1:1000
- ICC: 1:500 – 1:1000
Product Specifications
| Shipping | Dry Ice |
| Storage |
-20°C |
| Storage Information | Avoid freeze/thaw cycles |
| Species | Rabbit |
| Purity | The antibody was isolated from ascitic fluid by immunoaffinity chromatography using antigens coupled to agarose beads. |
| Form | Liquid |
| Isotype | IgG |
| Description | Rabbit Anti-Mouse IgG (H&L) conjugated to HRP. |
| Concentration | 1 mg/mL |
| Clonality | Polyclonal |
| Specificity | By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG. No antibody was detected against non immunoglobulin serum proteins. |
| Immunogen | Mouse IgG. |
| Buffer | Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.05% Sodium Azide. |
| Reactivity | Human |
