Product Description
Streptavidin is a tetrameric protein derived from Streptomyces avidinii with an exceptionally high affinity for biotin (Kd ≈ 10⁻¹⁴ mol/L). This strong and specific interaction forms the basis for a wide range of biochemical and diagnostic applications, enabling the detection, purification, and immobilization of biotinylated molecules.
The Strep-tag system leverages this binding specificity by incorporating a short peptide tag into recombinant proteins, allowing for efficient purification and detection through streptavidin- or Strep-Tactin–based methods.
This polyclonal antibody specifically recognizes the Strep-tag epitope and is optimized for Western blotting, providing sensitive and reliable detection of Strep-tagged proteins in research workflows.
Applications
- WB: 1:2000 – 1:10000
Product Specifications
| Shipping | Dry Ice |
| Storage |
-20°C |
| Storage Information | Avoid freeze/thaw cycles |
| Species | Rabbit |
| Purity | Antigen affinity purification. |
| Form | Liquid |
| Isotype | IgG |
| Description | The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin. |
| Concentration | 1.1 mg/mL |
| Clonality | Polyclonal |
| Specificity | Recognizes Strep tag in fusion proteins. |
| Immunogen | A synthetic peptide GASWSHPQFEKG conjugated to KLH. |
| Buffer | PBS, 50% glycerol, 1 mg/mL BSA, 1% Procolin300 |
| Reactivity | ALL |
