Product Description
Phenyl Hoperose FF (LS) is a fast flow, hydrophobic interaction chromatography (HIC) resin engineered for the purification and polishing of proteins, peptides, and other biomolecules under mild, non-denaturing conditions. Built on a 6% crosslinked agarose matrix functionalized with low-substitution phenyl ligands, it offers balanced hydrophobicity for selective separations, excellent product recovery, and superior reproducibility. The Fast Flow (FF) matrix ensures mechanical stability and scalability across laboratory and industrial bioprocessing.
Applications
- Intermediate purification and polishing of: Monoclonal antibodies (mAbs)
- Recombinant proteins
- Enzymes and plasma proteins
- Removal of hydrophobic aggregates or impurities following ion-exchange chromatography
Product Specifications
| Format | Liquid |
| Shipping | Gel Packs |
| Storage Solutions | 20% ethanol |
| Matrix | 6% crosslinked agarose (Fast Flow grade) |
| Ligand Density | High (strong HIC interaction) |
| Particle Size | 45–165 µm (avg. 90 µm) |
| Dynamic Binding Capacity | ≥40 mg BSA/mL resin |
| Functional Group | Phenyl (Low Substitution, LS) |
| pH Stability | 3–13 (operation), 2–14 (CIP) |
| Pressure Limit | ≤3 bar |
| Chemical Stability | 1 M NaOH, 6 M urea, 20% ethanol |
| Autoclave Stability | 121°C for 20 minutes |
| Storage Temperature | 2–8°C |
