Product Description
Receptor Activator of Nuclear Factor Kappa-B Ligand (RANKL) is a key TNF superfamily cytokine essential for osteoclast differentiation, bone remodeling, and immune regulation. RANKL functions as a soluble or membrane-associated homotrimer that binds the RANK receptor to activate downstream pathways, including AKT-mediated survival signaling. Its activity is naturally modulated by the decoy receptor OPG.
ORF Biologics’ recombinant RANKL with a C-terminal His tag is engineered for efficient affinity purification, improved handling, and straightforward detection in biochemical assays. This biologically active protein is produced to deliver high purity, structural integrity, and reliable batch-to-batch performance. It is ideal for studies involving osteoclastogenesis, bone biology, immune signaling, and TNF superfamily receptor–ligand interactions.
Applications
- Osteoclast Differentiation - Induces osteoclast formation and activity from precursor cells in vitro
- Bone Metabolism Research - Supports studies of bone remodeling, osteoporosis, and related disorders
- Immune Regulation - Enables investigation of RANKL-mediated T cell activation and dendritic cell survival
Product Specifications
| Storage | -20°C |
| Storage Information | Avoid freeze/thaw cycles |
| Species | Human |
| Amino Acid Sequence | EKAMVDGSWL DLAKRSKLEA QPFAHLTINA TDIPSGSHKV SLSSWYHDRG WAKISNMTFS NGKLIVNQDG FYYLYANICF RHHETSGDLA TEYLQLMVYV TKTSIKIPSS HTLMKGGSTK YWSGNSEFHF YSINVGGFFK LRSGEEISIE VSNPSLLDPD QDATYFGAFK VRDIDHHHHH H |
| Molecular Weight | 20.4 kDa (Expected) |
| Class | Recombinant |
| Purity | > 95% by SDS-PAGE gel |
| Endotoxin Concentration | < 1 EU/µg |
| Biologically Active | Yes |
| Biological Activity | The activity of RANKL is determined by its dose-dependent ability to induce reporter gene in HT-29 NF-κB Luc reporter cells. |
| Form | Lyophilized |
| Shipping Conditions | Ambient |
| Reconstitution | Reconstitute with sterile PBS. |
