Product Description
Vascular Endothelial Growth Factor 165 (VEGF 165) is a major isoform of the VEGF-A family and a key mediator of angiogenesis, vasculogenesis, and endothelial cell function. It exerts its biological activity by binding to VEGF receptors (VEGFR-1 and VEGFR-2) in the presence of co-receptors such as neuropilin-1, initiating downstream signaling cascades including the MAPK, PI3K/AKT, and PLCγ pathways. These pathways regulate endothelial cell proliferation, migration, survival, and new blood vessel formation.
VEGF 165 is abundantly expressed during embryonic development and in response to tissue injury or hypoxia, where it plays a vital role in establishing and remodeling the vascular system. In adult tissues, it continues to support angiogenesis, vascular permeability, and tissue regeneration. Recombinant VEGF 165 is widely used in vitro for endothelial cell culture, angiogenesis assays, and tissue engineering. It is also a valuable tool in cancer biology, wound healing studies, and the development of pro- and anti-angiogenic therapeutics in regenerative medicine and disease modeling.
Applications
- Endothelial cell proliferation and migration assays
- Angiogenesis studies in vitro and in vivo, including tube formation and sprouting models
- Vasculogenesis and vascular remodeling during development and regeneration
- Wound healing and tissue repair research
- Cancer biology, including tumor angiogenesis and metastasis models
- Hypoxia response and ischemia models for cardiovascular research
- Tissue engineering and biomaterial vascularization
- Retinal and ocular disease studies involving neovascularization
Product Specifications
| Storage | -20°C |
| Storage Information | Avoid freeze/thaw cycles |
| Species | Human |
| Amino Acid Sequence | APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR |
| Molecular Weight | 19.2 kDa (Expected) |
| Class | Recombinant |
| Purity | > 95% by SDS-PAGE gel |
| Endotoxin Concentration | < 1 EU/µg |
| Biologically Active | Yes |
| Biological Activity | The biological activity is confirmed by its ability to stimulate the proliferation of human umbilical vein endothelial cells (HUVECs). |
| Form | Lyophilized |
| Shipping Conditions | Ambient |
| Reconstitution | Reconstitute with sterile 10 mM Acetic Acid + 0.1% BSA |
