Product Description
SOLIScript® 1-step Multiplex Probe Kit
Description
SOLIScript® 1-step Multiplex Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube.
Reagent composition is optimized for excellent performance in highly multiplexed reactions while making sure you don't lose in sensitivity compared to singleplex or duplex assays.
Applications
- Detection and quantification of RNA targets with qPCR
- DNA/LNA hydrolysis probe based assays
- Suitable for singleplex and multiplex reactions
Properties
Sample type: RNA
Product format: Reagent kit
Detection type: Probe-based
Reference dye: none
Compatible real-time instruments: The kit is compatible with qPCR cyclers that do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal. Check Your cycler!
Reagents
5x One-step Multiplex Probe Mix with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP, 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2)
40x One-step SOLIScript® Mix with SOLIScript® Reverse Transcriptase, RiboGrip® RNase Inhibitor
Water, nuclease free
Storage
Routine storage: -20 ºC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
Shipping
At room temperature
|
Item |
Pcs. |
Vial size |
|
40x One-step SOLIScript® Mix |
1 |
250 rxn | 125 µl |
|
5x One-step Multiplex Probe Mix |
1 |
250 rxn | 1 ml |
|
Water, nuclease free |
1 |
5 ml |
