SOLIScript® 1-step Multiplex Probe Kit (Purple)

Product Description

SOLIScript® 1-step Multiplex Probe Kit (Purple)

Description

SOLIScript^® 1-step Multiplex Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube.

Reagent composition is optimized for excellent performance in highly multiplexed reactions while making sure you don't lose in sensitivity compared to singleplex or duplex assays.

Applications

•  Detection and quantification of RNA targets with qPCR
• DNA/LNA hydrolysis probe based assays
• Suitable for singleplex and multiplex reactions

Properties

Sample type: RNA

Product format: Reagent kit 

Detection type: Probe-based

Reference dye: Purple

Compatible real-time instruments: 7500 Fast Real-Time PCR System, 7500 Real-Time PCR System, ViiA™ 7 PCR System, and QuantStudio™ 6 Flex, 7 Flex, and 12K Flex Real-Time PCR systems.

Reagents 

5x One-step Multiplex Probe Mix (Purple) with HOT FIREPol^® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl₂ (1x qPCR solution - 3 mM MgCl₂), Purple dye (If Cy5 dye is used as one of the fluorophores, internal passive reference dye might interfere with the signal. A version without passive reference or with ROX reference dye is available)

40x One-step SOLIScript^® Mix with SOLIScript^® Reverse and Transcriptase and RiboGrip^® RNase Inhibitor

Water, nuclease free

Storage

Routine storage: -20 ºC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.

Shipping

At room temperature