SOLIScript® 1-step Probe Kit

Product Description

SOLIScript® 1-step Probe Kit

Description

SOLIScript^® 1-step Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays.

It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube

Applications

• Detection and quantification of RNA targets with qPCR
• DNA/LNA hydrolysis probe based assay
• Suitable for singleplex or duplex reactions

Properties

Sample type: RNA

Product format: Reagent kit 

No of tubes: three tubes

Detection type: Probe-based

Reference dye: reference dye based on ROX

Compatible real-time instruments: The  Kit is compatible with most qPCR cyclers that do or do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal.

Reagents 

5x One-step Multiplex Probe Mix (ROX) with HOT FIREPol^® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl₂ (1x qPCR solution - 3 mM MgCl₂), internal reference based on ROX dye

40x One-step SOLIScript^® Mix with SOLIScript^® Reverse Transcriptase and RiboGrip® RNase Inhibitor

Water, nuclease free

Storage

Routine storage: -20 ^oC

Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of SOLIScript^® 1-step Probe Kit.

Shipping

At room temperature.