SOLIScript® 1-step SolisGreen® Kit 2.0

Product Description

SOLIScript® 1-step SolisGreen® Kit 2.0

Description

SOLIScript® 1-step SolisGreen® Kit 2.0 is specially designed for dye-based real-time one-step RT-PCR (RT-qPCR) assays. SolisGreen® dsDNA binding dye is incorporated into the product to evaluate the DNA amplification process during qPCR. The fluorescence spectra of this dye are similar to the more widely used SYBR® Green I and is compatible with most major real-time cyclers. When compared to SYBR® Green I, SolisGreen® dye shows:

• higher fluorescence level
• better sensitivity for detecting low template concentrations
• great stability for room temperature storage

SOLIScript® 1-step SolisGreen® Kit 2.0 is an optimized ready-to-use kit that comes in 3 tubes (including nuclease free water) and contains all the necessary components, except sample (RNA template) and primers, to perform reactions with accurate and highly sensitive results. Additionally, the product includes a passive reference based on ROX dye making it compatible with both ROX-dependent and ROX-independent qPCR cyclers.

Applications

• Gene expression analysis and absolute quantification
• Pathogen detection and quantification
• Low-copy gene detection
• miRNA profiling and quantification

Properties

Sample type: RNA

Product format: Reagent kit (nuclease free water supplied in a separate tube)

Detection type: Dye-based, includes SolisGreen® intercalating dye

Reference dye: Based on ROX

Compatible real-time instruments: The mix is compatible with both ROX-dependent and ROX-independent qPCR cyclers. SOLIScript® 1-step SolisGreen® Kit 2.0 is not compatible with platforms that require high ROX, such as Applied BioSystems® 7900HT, StepOne™ or StepOnePlus™ systems.

Kit Components

40x One-step SOLIScript® Mix: SOLIScript® Reverse Transcriptase, RiboGrip® RNase Inhibitor.

5x One-step SolisGreen® Mix 2.0: HOT FIREPol® DNA polymerase, dNTPs (dATP, dCTP, dGTP, dTTP), 12.5 mM MgCl₂ (1x RT-qPCR solution – 2.5 mM MgCl₂), SolisGreen® dye, passive reference dye based on ROX dye.

Water, nuclease-free.

Storage

Routine storage: -20 ºC

Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.

Shipping

At room temperature.