Product Description
SOLIScript® RT cDNA synthesis KIT
Description
SOLIScript® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase.
SOLIScript® RT can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatuures (up to 60°C).
SOLIScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a RNase H domain with reduced activity.
Applications
- First strand cDNA synthesis
- RT-PCR
- RT-qPCR
Properties
Sample type: RNA
Final product: cDNA (first strand)
Product format: Enzyme with all components included reagent kit
Enzyme: SOLIScript® Reverse Transcriptase
Source: Purified from an E. coli strain that carries an overproducing plasmid containing a SOLIScript® Reverse Transcriptase gene.
Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Reagents
SOLIScript® Reverse Transcriptase
RiboGrip® RNase Inhibitor (220 U/μl)
10x RT Reaction Buffer with DTT, 500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT
Oligo (dT) Primer (100 μM)
dNTP MIX (20 mM of each)
Water, nuclease free
NB! A component of this kit has changed. RiboGrip® RNase Inhibitor (Cat. No. 06-22) has been discontinued and is replaced by a new product: RiboGrip® RNase Inhibitor 220 U/µl. Please refer to the product data sheet for up-to-date usage instructions.
Storage
Routine storage: -20oC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
Shipping
At room temperature.
| Item | Pcs. | Vial size |
| SOLIScript® Reverse Transcriptase | 1 | 50 rxn | 50 µl / 200 rxn | 200 µl |
| RiboGrip® RNase Inhibitor (220 U/µl) | 1 | 2000 U | 9 μl / 4000 U | 18 μl |
| 10x RT Reaction Buffer with DTT | 1 | 125 µl / 500 µl |
| dNTP MIX (20 mM of each) | 1 | 2 µmol | 25 µl / 8 µmol |
| Oligo (dT) Primer | 1 | 50 µl / 200 µl |
| Water, nuclease free | 1 / 3 | 1.25 ml |
