SOLIScript® RT cDNA synthesis KIT

Product Description

SOLIScript® RT cDNA synthesis KIT

Description

SOLIScript^® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase.

SOLIScript^® RT can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatuures (up to 60°C).

SOLIScript^® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a RNase H domain with reduced activity.

Applications

• First strand cDNA synthesis
• RT-PCR
• RT-qPCR

Properties

Sample type: RNA

Final product: cDNA (first strand)

Product format: Enzyme with all components included reagent kit

Enzyme: SOLIScript^® Reverse Transcriptase

Source: Purified from an E. coli strain that carries an overproducing plasmid containing a SOLIScript^® Reverse Transcriptase gene.

Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Reagents

SOLIScript^® Reverse Transcriptase

RiboGrip^® RNase Inhibitor (220 U/μl)

10x RT Reaction Buffer with DTT, 500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT

Oligo (dT) Primer (100 μM)

dNTP MIX (20 mM of each)

Water, nuclease free

NB! A component of this kit has changed. RiboGrip® RNase Inhibitor (Cat. No. 06-22) has been discontinued and is replaced by a new product: RiboGrip® RNase Inhibitor 220 U/µl. Please refer to the product data sheet for up-to-date usage instructions.

Storage

Routine storage: -20^oC

Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.

Shipping

At room temperature.