Product Description
SOLIScript® RT cDNA synthesis MIX
Description
SOLIScript® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase.
SOLIScript® RT can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatuures (up to 60°C).
SOLIScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a RNase H domain with reduced activity.
Applications
- First strand cDNA synthesis
- RT-PCR
- RT-qPCR
Properties
Sample type: RNA
Final product: cDNA (first strand)
Product format: Enzyme mix with all reagents included
Enzyme: SOLIScript® Reverse Transcriptase
Source: Purified from an E. coli strain that carries an overproducing plasmid containing a SOLIScript® Reverse Transcriptase gene.
Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Reagents
SOLIScript® Enzyme Mix with SOLIScript® RT and RiboGrip® RNase Inhibitor
10x RT Reaction Premix without primers, RT reaction buffer with DTT and dNTPs
Water, nuclease free
Storage
Routine storage: -20oC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
Shipping
At room temperature.
|
Item |
Pcs. |
Vial size |
|
SOLIScript® Enzyme Mix |
1 |
100 rxn | 150 µl |
|
10x RT Reaction Premix without primers |
1 |
250 µl |
|
Water, nuclease free |
2 |
1.25 ml |
