Product Description
SolisFAST® Probe qPCR Mix (no ROX)
Description
SolisFAST® Probe qPCR Mix is a 5x-concentrated, ready-to-use solution for fast, highly sensitive and reproducible probe-based qPCR assays using dual-labeled hydrolysis probes (e.g. TaqMan® probes), and is suitable for detection and quantitation of up to five targets simultaneously.
Combining our novel in silico- designed SolisFAST® DNA Polymerase and optimized buffer, this mix offers robust qPCR, and accurate target detection combined with ice free shipping and reaction set-up.
Applications
- DNA/LNA hydrolysis probe based assays
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination
Properties
Concentration: 5x
Hot-start: yes, 3 minutes initial incubation at 95 °C
Cycling speed: fast or ultra-fast
Detection type: Probe-based
Reference dye: none
Multiplex: up to 5 plexes simultaneously
Compatible real-time instruments: The mix is compatible with qPCR cyclers that do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal.
Mix Components:
SolisFAST® DNA Polymerase: In silico- designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.
qPCR buffer: Includes 15 mM MgCl2 (1x PCR solution 3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), additives that maximize efficiency of PCR
Routine storage at -20 °C until expiry date
The mix can be stored at +4 °C for up to 1 year
Stability at room temperature (15 − 25 °C) for 6 months
Freeze-thaw stability: 30 cycles
At room temperature
