Product Description
SolisFAST® Probe qPCR Mix with UNG (no ROX)
Description
SolisFAST® Probe qPCR Mix is a 5x-concentrated, ready-to-use solution for fast, highly sensitive and reproducible probe-based qPCR assays using dual-labeled hydrolysis probes (e.g. TaqMan® probes), and is suitable for detection and quantitation of up to five targets simultaneously.
Combining novel in silico-designed SolisFAST® DNA Polymerase with fast hot-start and optimized buffer, this qPCR Mix offers accurate target detection and quantification.
For extra convenience the mix includes dUTP and UNG enzyme that enable UNG treatment to prevent carryover contamination.
This master mix is developed for TaqMan® probes but is also suitable for other hydrolysis probe types.
Applications
- DNA/LNA hydrolysis probe based assays
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination
Properties
Concentration: 5x
Hot-start: yes, 3 minutes initial incubation at 95 °C
Cycling speed: fast or ultra-fast
Detection type: Probe-based
Reference dye: none
Multiplex: up to 5 plexes simultaneously
Compatible real-time instruments: The mix is compatible with qPCR cyclers that do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal.
Mix Components:
SolisFAST® DNA Polymerase: In silico- designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.
qPCR buffer: Includes 16.5 mM MgCl2 (1x PCR solution 3.3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dUTP), UNG, additives that maximize efficiency of PCR
Storage
Routine storage at -20 °C until expiry date
The mix can be stored at +4 °C for up to 1 year
Stability at room temperature (15 - 25 °C) for 3 months
Freeze-thaw stability: 30 cycles
Shipping
At room temperature
