Product Description
SolisFAST® SolisGreen® qPCR Mix (no ROX)
Description
SolisFAST® SolisGreen® qPCR Mix is a 5x-concentrated ready-to-use solution optimized for fast, highly sensitive and reproducible dye-based qPCR assays.
Combining novel in silico-designed SolisFAST® DNA Polymerase with fast hot-start, optimized buffer and the SolisGreen® dye, this qPCR Mix offers robust qPCR, accurate target detection, ice free shipping and reaction set-up.
Applications
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination
Properties
Concentration: 5x
Hot-start: yes, 3 minutes initial incubation at 95°C
Cycling speed: fast and ultra-fast
Detection type: dye-based, includes SolisGreen® intercalating dye
Reference dye: none
Compatible real-time instruments: Most qPCR cyclers that do not require reference dye.
Mix Components:
SolisFAST® DNA Polymerase: In silico- designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.
qPCR buffer: Includes 7.5 mM MgCl2 (1X PCR solution 1.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), stabilizers and enhancers that maximize efficiency of PCR reaction
Fluorescent dye: SolisGreen® is a nucleic acid dye detected in the FAM or SYBR® Green I channel
SolisGreen® dye
This dye-based qPCR mix contains next generation DNA binding dye SolisGreen®. The fluorescence spectra of this dye is similar to the more widely used SYBR® Green I and is compatible with most major realtime cyclers. SolisGreen® dye shows:
- high fluorescence level
- high sensitivity for detecting low template concentrations
- high stability for room temperature storage
