Product Description
MagaBio plus General Genomic DNA Purification Kit Ⅱ is used for extraction and purification of genomic DNA from human whole blood, tissue, saliva, oral swabs and dry blood spots specimens. The purified nucleic acid is suitable for clinical in vitro testing or use in amplification-based in vitro diagnostic assays.
Diagnostic results obtained using the nucleic acid purified with the kit must be interpreted in conjunction with other clinical or laboratory data. The kit is intended to be used by professional users, such as technicians or physicians who are trained in molecular biological techniques.
For professional use only. For in vitro diagnostic use only.
PRINCIPLE OF THE PROCEDURE
DNA in the sample is released by PK Solution and Lysis Buffer. Released DNA is bound exclusively and specifically to the MagaBio Reagent. DNA bound to Magnetic particles are captured by a magnetic tool and contaminants are removed by Wash Buffer once or more. The DNA is then eluted from particles by Elution Buffer or molecular grade water.
KIT COMPONENTS
| Catalog | BSC74S1B | BSC74M1B |
| Kit size (Tests/Kit) | 50 | 100 |
| TES Buffer | 25 mL | 50 mL |
| PK Solution | 500 µL | 1 mL |
| RNase A Solution | 100 µL | 200 µL |
| Lysis Buffer | 20 mL | 40 mL |
| Wash Buffer I | 16 mL a | 32 mLc |
| Wash Buffer II | 18 mL b | 18 mLd×2 |
| Elution Buffer | 10 mL | 20 mL |
| MagaBio Reagent | 1.5 mL | 3 mL |
| Instructions for Use | 1 | 1 |
Notes:
1. The components of different lots cannot be mixed for use.
2. For BSC74S1B, a. Add 24mL Absolute ethanol before use; b. Add 42mL
Absolute ethanol before use.
For BSC74M1B, c. Add 48mL Absolute ethanol before use; d. Add 42mL
Absolute ethanol before use.
EQUIPMENT AND MATERIALS REQUIRED BUT NOT PROVIDED
1. Analytical grade absolute ethanol, magnetic rack, Water bath or dry bath,
Vortex mixer, Centrifuge;
2. Centrifuge tubes and micropipettes, micropipette tips;
3. Disposable gloves, etc
STORAGE AND TRANSPORTATION
RNase A Solution should be stored 2 ~8℃, other components of the kit should be
stored at 2 ~25℃ for the duration of the shelf life.
Do not use beyond the expiration date printed on the box.
The kit can be transported at room temperature.
The kit can be stored for up to 12 months if all components are kept on the
conditions above.
