Product Description
MagaBio plus General Genomic DNA Purification Kit II is designed for rapid and reliable isolation of high-quality genomic DNA from blood, tissue, saliva, buccal swabs, cultured cells, and dry blood spots.
MagaBio Particles, magnetic beads, provide a quick magnetic response time reducing overall processing time. This system combines the reversible nucleic acid-binding properties of MagaBio paramagnetic particles with the time-proven efficiency of Bioer automatic nucleic acid purification system to provide a fast and convenient method to isolated DNA from a variety of samples. Utilizing paramagnetic particles provides high-quality DNA that is suitable for direct use in most downstream applications, such as amplification and enzymatic reactions, next-generation sequencing
PRINCIPLE OF THE PROCEDURE
Nucleic acid is released under the action of Proteinase K Solution and Lysis Buffer. Magnetic beads can
combine with DNA exclusively and specifically after adding isopropanol. Magnetic beads combined with DNA
are captured by magnetic rod; Multiple Wash Buffers will remove contaminants. Purified DNA is released into
the Elution Buffer by Magnetic beads.
MagaBio technic has great advantages:
1. Mini sample, high purification;
2. Simple and streamline separation procedure, used for auto-platform;
3. First elution can acquire 85% or more DNA;
4. No high salt solution. No inhibitor;
5. No spin column.
KIT COMPONENTS
| Catalog | BSC74S1E | BSC74M1B |
| Kit size (Tests/Kit) | 32 | 100 |
| TES Buffer | 16 mL | 50 mL |
| PK Solution | 320 µL | 1 mL |
| RNase A Solution | 32 µL | 200 µL |
| Lysis Buffer | 96 well pre-packed plate 2 pieces | 40 mL |
| Wash Buffer I | 32 mLc | |
| Wash Buffer II | 18 mLd×2 | |
| Elution Buffer | 20 mL | |
| MagaBio Reagent | 3 mL | |
| Instructions for Use | 1 | 1 |
EQUIPMENT AND MATERIALS REQUIRED BUT NOT PROVIDED
1. Magnetic Rack or Bioer NPA-32P purification instrument;
2. Water bath or Dry bath;
3. Vortex mixer;
4. Absolute alcohol (For BSC74S1B and BSC74M1B).
STORAGE AND TRANSPORTATION
1. The kit can be transported at room temperature.
2. All reagents are stored at 2-8℃.
3. All reagents, when stored properly, are stable for 12 months from the time of delivery
