Biospin Viral DNA/RNA Extraction Kit 100 Prep

Product Description

Biospin Virus Nucleic acid Extraction Kit

Introduction

BioSpin Viral Nucleic acid extraction kit is fast and convenient way for rapid isolation of DNA or RNA for viral pathogens, and recently have been validate for several strains of SARS-CoV-2, agent of the Covid-19 diseases. Universal regarding samples Biospin work with swabs, plasma (fresh or frozen), serum, urine, saliva, feces and virus-infected cell cultures or tissues. The purified nucleic acids are ready for PCR, RT-PCR, NGS and any other existing or upcoming application.

BioSpin advantages

• Viral NA extracted in 25-30 minutes
• High sensitivity , 10 IU/ml from DNA or 100 IU/ml from RNA pathogens
• Universal, extract nucleic acid from virus , bacterial and fungus , from different biological samples
• Phenol free

Kit Contents

Storage and transportation

1. The kit can be transported at room temperature.
2. The kit has demonstrated stability of 12 months when stored at room temperature. Proteinase K stored at 2-8℃

Equipment and reagents to be supplied by user

1. Microcentrifuge capable of 14,000rpm
2. Metal bath or Water bath
3. Vortex mixer
4. Absolute ethanol (AR)

Important Notes

1)    Lysis Buffer may be precipitated at low temperature, please heated at 56 ℃ for a few minutes to restore the clarification.

2)    Wash Buffer I and Wash Buffer II add the absolute ethanol as the volume marked on bottle label and mix well.

Sample Requirements

If the liquid sample volume is less than 200μL, you can add PBS or normal saline to make the total volume to 200μL.

Kit Components

PS：Buy BSC77S1    add 12mL Absolute ethanol to ※18mL Wash Buffer I before use. add 48mL Absolute ethanol to *12mL Wash buffer II before use.

Buy BSC77M1   add 24mL Absolute ethanol to ※36mL Wash Buffer I before use. add 96mL Absolute ethanol to *24mL Wash buffer II before use.

Buy BSC77L1   add 48mL Absolute ethanol to ※72mL Wash Buffer I before use. add 192mL Absolute ethanol to *48mL Wash buffer II before use.

Procedure

Ⅰ Sample pretreatment

Animal/Plant  Tissue:  Grind  sample  fully  with  normal  saline  or PBS,  take  the  supernatant  after centrifugation.

Serum, Plasma, Ascites and other liquid samples: Extraction directly.

Fecal: Add an appropriate amount of normal saline or PBS into the samples, shake them thoroughly, centrifuge them at 12000g for 5min, and take the supernatant for extraction.

Ⅱ Sample extraction operation

1. Pipet 10μL PK into a 1.5 mL microcentrifuge tube (not provided).
2. Add 200μL sample (If the sample volume ≤200 μL, replenish PBS or normal saline to a volume of 200μL) into the microcentrifuge tube.
3. Add 200μL Lysis Buffer. Vortex mixing 30 second
4. Incubate at 56°C for 15 min in a heating block. Briefly centrifuge the 1.5 mL tube to remove drops from the inside of the tube lid.
5. Add 250  μL  of  ethanol  (96–100%)  to  the  sample,  close  the  cap  and  mix  thoroughly  by pulse-vortexing for 15 Briefly centrifuge the 1.5 mL tube to remove drops from the inside of the tube lid.
6. Transfer the mixture into a Spin Column, centrifuge at 10,000 g for 1 minute and discard the flow-through.
7. Add 500μL Wash Buffer I into the Spin Column, centrifuge at 10,000 g for 1 minute and discard the flow-through.
8. Add 500μL Wash Buffer II into the Spin Column, centrifuge at 10,000 g for 1 minute and discard the flow-through.
9. Add 500μL Wash Buffer II into the Spin Column, centrifuge at 10,000 g for 1 minute and discard the flow-through.
10. Place the spin column in a clean 1.5 mL collection tube. Centrifuge at 10,000 g for 2 min to dry the membrane completely.
11. Place the spin column in a clean 1.5 mL collection tube. Add 50-100 μL Elution Buffer (or RNase-free water pH>7.0) to the central of the membrane; Incubate at the room temperature for 2 minutes.
12. Centrifuge at 12,000 g for 1 minu Remove the Spin Basket and discard. Then the buffer in the microcentrifuge tube contains the DNA/ RNA