Product Description
The kit provides a simple, fast and cost effective method that can isolate high quality DNA. Using one simple protocol, high yield purified DNA can be isolated from various samples including animal tissue, whole blood, buffy coat, leukocytes, bacteria, yeast, blood spots, swabs and cultured cells. The kit utilizes silica-based membrane techniques within a convenient spin column, no need of expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The whole procedure takes less than 20 minutes including lysis steps, and more than 20 kb DNA could be purified by this kit. Isolated DNA can be directly applied to PCR, Southern blotting and other enzymatic reactions.
Kit Components
| Cat# | BSC39M1 |
| Components | 100 Tests |
| TES buffer | 28mL |
| TET buffer | 18mL |
| LysisB buffer | 50mL |
| PK solution | 1mL×2 |
| RNase A | 400μL |
| PW Buffer | 24mL (add 36mL absolute ethanol) |
| Wash Buffer | 48mL (add 72mL absolute ethanol) |
| Elution Buffer | 10mL |
| Spin Columns | 100 |
| Handbook | 1 |
Storage & shipping conditions
1. Store Protease K and RNase A at 2~8℃,store all other components at room temperature.
2. All components are stable for 18 months from date of receipt under proper storage condition.
Addtional apparatus and materials required but not supplied
* Sterile 1.5mL microcentrifuge tubes * 10μL/200μL/1000μL tips
* Centrifuge capable of 12,000g * Absolute ethanol
* Vortex mixer * Warm bath
* PBS buffer * Lysozyme
* Lyticase * Sorbitol buffer (18.217g sorbitol + 3.7224g
EDTA-2Na, dissolve into 100 mL ddH2O)
