Product Description
Introducing the Biospin Plasmid DNA Extraction Kit, a versatile solution designed to cater to varying sample volumes, available in three convenient formats: Mini, Midi, and Maxi. Harnessing the efficiency of spin column technology, this kit offers streamlined plasmid DNA extraction workflows tailored to your specific experimental needs.
Experience efficient plasmid DNA extraction tailored to your sample volume requirements with the Biospin Plasmid DNA Extraction Kit. Whether you're processing small, medium, or large volumes of bacterial cultures, our kit provides a reliable and convenient solution for your research needs.
The kit provides a fast, simple, and cost-effective plasmid miniprep method for routine molecularbiology laboratory applications. Plasmid DNA can be purified from 1-5ml. of overnight cultures of E. coli. The DNA isolated by this kit is ready fo downstream applications such as restriction enzyme digestion, sequencing. PCR/Real-time PCR and other downstream experiments.
Product Features
- Scalable Formats: Choose from Mini, Midi, and Maxi formats to accommodate different sample volumes, ensuring flexibility and convenience for a wide range of applications.
- Spin Column Method: Utilizing centrifugation-based purification, our kit efficiently isolates plasmid DNA from bacterial cultures, delivering high yields and purity with minimal hands-on time.
- Reliable Performance: Benefit from consistent and reproducible results across all three formats, enabling reliable extraction of high-quality plasmid DNA for downstream applications.
- Easy-to-Follow Protocols: With user-friendly protocols provided for each format, our kit simplifies the DNA extraction process, making it accessible to researchers of all experience levels.
- Compatibility: Compatible with various sample types and bacterial strains commonly used in molecular biology research, ensuring versatility and applicability to diverse experimental setups.
Application Fileds
- Molecular cloning
- Gene expression analysis
- Transfection studies
- Sequencing and genotyping
- Protein production and purification
Storage Conditions
The kit should be stored at room temperature(15-25°C), but the RNase solution should bestored at 2-8°C.
The kit can be stored for up to 18 months by this method. After addition of RNase solution, Resuspension Buffer should be stored 2-8°C.
The kit can be transported at room temperature.
Addtional apparatus and materials required but not supplied
* Sterile 1.5mL microcentrifuge tubes * 10μL/200μL/1000μL tips
* Centrifuge capable of 12,000g * Absolute ethanol
* Vortex mixer * Warm bath
* PBS buffer * Lysozyme
* Lyticase * Sorbitol buffer (18.217g sorbitol + 3.7224g
EDTA-2Na, dissolve into 100 mL ddH2O)
