Product Description
The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from plant tissues, adopting the Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA less than 1 hour. It not requires expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, 1-30 μg genomic DNA can be acquired from up to 100 mg tissue by using this kit.
The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.
Principle
At first, the plant tissue is lysed in LP buffer, and then DNA in the sample is liberated. After adding DA Buffer and centrifuging, the impurity will be discarded. Released DNA is bound exclusively and specifically to the Biospin membrane in presence of a P Binding Buffer under appropriate salt iron and pH conditions. Denatured protein and other contaminants are removed by several washing procedures. The DNA is then eluted from the membrane with the Elution Buffer.
Kit Components
| Cat# | BSC13S1B |
| Components | 50 Tests |
| LP Buffer | 22.5 mL |
| LP plus Buffer | 22.5 mL |
| DA Buffer | 7.5 mL |
| P Binding Buffer | 14mL |
| G Binding Buffer | 25mL |
| Wash Buffer | 25.2mL |
| Elution Buffer | 10mL |
| Spin columns | 50 |
| Shredder Spin Column | 50 |
| Handbook | 1 |
Storage & shipping conditions
- The kit should be stored at 15-25℃.
- All reagents, when stored properly, are stable for 18 months.
Addtional apparatus and materials required but not supplied
- Sterile 1.5mL microcentrifuge tubes
- 10μL/200μL/1000μL tips
- Centrifuge capable of 12,000g
- Absolute ethanol
- Vortex mixer
