Milenia HybriDetect LAMP Hot Start Kit - Isothermal Amplification Kit (100 Reactions)

Product Description

Milenia Biotec HybriDetect LAMP Hot Start Kit
High-Specificity Isothermal Amplification for Lateral Flow

The Milenia Biotec HybriDetect LAMP Hot Start Kit is a comprehensive reagent set designed for the simplified and specific amplification of DNA/RNA using Loop-mediated Isothermal Amplification (LAMP). The key feature of this kit is the inclusion of a Hot Start Bst Polymerase, which is reversibly inhibited by an aptamer at temperatures below 45°C. This allows researchers to set up reactions at room temperature without non-specific amplification or primer-dimer formation, ensuring high specificity and ease of handling.

Optimized for Lateral Flow Detection

Unlike standard LAMP master mixes, this kit is specifically formulated to generate labeled amplicons (Biotin/FITC) that are perfectly compatible with Milenia HybriDetect lateral flow strips (Dipsticks or Cassettes). The reaction buffer is optimized to minimize background noise on the strip, allowing for a seamless transition from amplification to visual readout in minutes.

Key Features

• Hot Start Technology: Aptamer-based inhibition prevents activity at room temperature, reducing non-specific background.
• High Specificity: Ideal for complex templates or low-copy targets.
• Room Temperature Setup: No need for ice blocks during reaction preparation.
• Rapid Amplification: Results in 15 – 60 minutes at constant temperature (60-65°C).
• Lateral Flow Ready: Optimized for use with HybriDetect universal test strips.

Technical Specifications