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    • PCR Reagent
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    • Milenia HybriDetect LAMP Hot Start Polymerase (HLHSP 1) - Isothermal Polymerase (100 Reactions)
  • Milenia Biotec HybriDetect LAMP Hot Start Polymerase (HLHSP 1) - Isothermal Polymerase (100 Reactions)
  • Milenia Biotec HybriDetect LAMP Hot Start Polymerase (HLHSP 1) - Isothermal Polymerase (100 Reactions)

    Milenia HybriDetect LAMP Hot Start Polymerase (HLHSP 1) - Isothermal Polymerase (100 Reactions)

    Milenia Biotec

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    Product Description

    Milenia Biotec HybriDetect LAMP Hot Start Polymerase (HLHSP 1)
    Flexible Enzyme for Custom LAMP Assay Development

    The Milenia Biotec HybriDetect LAMP Hot Start Polymerase (SKU: HLHSP 1) is a specialized enzyme kit designed for researchers who require full flexibility in developing and optimizing their own Loop-mediated Isothermal Amplification (LAMP) assays. Unlike ready-to-use master mixes, this kit provides the single components necessary to adjust reaction conditions specifically for challenging targets or unique primer sets. It features a Hot Start Bst Polymerase, which is reversibly inhibited by an aptamer at lower temperatures, effectively preventing non-specific amplification during reaction setup at room temperature.

    High Sensitivity and Lateral Flow Compatibility

    This polymerase is engineered to deliver exceptional sensitivity, capable of detecting as few as 3 molecules of target DNA. It is perfectly suited for generating labeled amplicons (e.g., using Biotin and FITC labeled primers) for subsequent visualization on Milenia HybriDetect universal lateral flow strips. This combination allows for a rapid, robust, and equipment-free readout, making it an ideal choice for the development of highly sensitive point-of-care diagnostics.

    Mechanism of Labeled LAMP Amplicon Detection on HybriDetect

    Key Features

    • Custom Assay Design: Single components allow for the fine-tuning of buffer and enzyme concentrations for specific assays.
    • Hot Start Function: Aptamer-based inhibition enables convenient reaction assembly at room temperature without background noise.
    • Ultra-High Sensitivity: Validated to detect down to 3 copies of the target DNA sequence.
    • Versatile Evaluation: Optimized for Lateral Flow strips (MGHD 1), but also compatible with Agarose Gel Electrophoresis and Fluorescence methods.
    • Seamless Workflow: Integrates perfectly with HybriDetect strips to provide visual results in minutes after amplification.

    Technical Specifications

    Parameter Specification
    Catalog Number HLHSP 1
    Enzyme Type Bst Polymerase (Hot Start / Aptamer blocked)
    Reactions 100 Reactions
    Sensitivity 3 molecules of target DNA
    Target Analyte DNA
    Readout Methods Lateral Flow (MGHD 1), Gel Electrophoresis, Fluorescence
    Compatible Products MGHD 1, MGHD2 1, MGHC 1, MYILABR 1

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    Milenia Biotec HybriDetect LAMP Hot Start Polymerase (HLHSP 1) - Isothermal Polymerase (100 Reactions)
    Milenia Biotec
    Milenia HybriDetect LAMP Hot Start Polymerase (HLHSP 1) - Isothermal Polymerase (100 Reactions)

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