Product Description
Milenia Biotec HybriDetect LAMP Hot Start Polymerase (HLHSP 1)
Flexible Enzyme for Custom LAMP Assay Development
The Milenia Biotec HybriDetect LAMP Hot Start Polymerase (SKU: HLHSP 1) is a specialized enzyme kit designed for researchers who require full flexibility in developing and optimizing their own Loop-mediated Isothermal Amplification (LAMP) assays. Unlike ready-to-use master mixes, this kit provides the single components necessary to adjust reaction conditions specifically for challenging targets or unique primer sets. It features a Hot Start Bst Polymerase, which is reversibly inhibited by an aptamer at lower temperatures, effectively preventing non-specific amplification during reaction setup at room temperature.
High Sensitivity and Lateral Flow Compatibility
This polymerase is engineered to deliver exceptional sensitivity, capable of detecting as few as 3 molecules of target DNA. It is perfectly suited for generating labeled amplicons (e.g., using Biotin and FITC labeled primers) for subsequent visualization on Milenia HybriDetect universal lateral flow strips. This combination allows for a rapid, robust, and equipment-free readout, making it an ideal choice for the development of highly sensitive point-of-care diagnostics.

Key Features
- Custom Assay Design: Single components allow for the fine-tuning of buffer and enzyme concentrations for specific assays.
- Hot Start Function: Aptamer-based inhibition enables convenient reaction assembly at room temperature without background noise.
- Ultra-High Sensitivity: Validated to detect down to 3 copies of the target DNA sequence.
- Versatile Evaluation: Optimized for Lateral Flow strips (MGHD 1), but also compatible with Agarose Gel Electrophoresis and Fluorescence methods.
- Seamless Workflow: Integrates perfectly with HybriDetect strips to provide visual results in minutes after amplification.
Technical Specifications
| Parameter | Specification |
|---|---|
| Catalog Number | HLHSP 1 |
| Enzyme Type | Bst Polymerase (Hot Start / Aptamer blocked) |
| Reactions | 100 Reactions |
| Sensitivity | 3 molecules of target DNA |
| Target Analyte | DNA |
| Readout Methods | Lateral Flow (MGHD 1), Gel Electrophoresis, Fluorescence |
| Compatible Products | MGHD 1, MGHD2 1, MGHC 1, MYILABR 1 |