Product Description
Milenia Biotec HybriDetect LAMP Kit (HLK 1)
Ready-to-Use Isothermal Amplification Mix
The Milenia Biotec HybriDetect LAMP Kit (SKU: HLK 1) is a streamlined, ready-to-use master mix designed to simplify the development of Loop-mediated Isothermal Amplification (LAMP) assays. This kit provides all the essential components (excluding primers and template) in a pre-optimized formulation, allowing researchers to amplify DNA targets rapidly under constant temperature conditions. It is specifically engineered to produce labeled amplicons that can be immediately detected using Milenia HybriDetect lateral flow strips.
Optimized for Lateral Flow Readout
While compatible with standard fluorescence and agarose gel electrophoresis, the buffer chemistry of the HLK 1 kit is fine-tuned to minimize background interference on lateral flow dipsticks. When used with biotin and FITC/FAM labeled primers, the resulting amplicons bind efficiently to the test strip, providing a clear visual result in minutes without the need for complex optical equipment.
Key Features
- Convenient Master Mix: Pre-mixed formulation reduces pipetting steps and potential errors.
- High Sensitivity: Capable of detecting as few as 5 molecules of target DNA.
- Rapid Isothermal Reaction: Amplification typically completes within 15 – 60 minutes at 60-65°C.
- Versatile Detection: Results can be visualized via Lateral Flow, Fluorescence, or Gel Electrophoresis.
- Cost-Effective Development: Ideal for establishing new LAMP assays for point-of-care applications.
Technical Specifications
| Parameter | Specification |
|---|---|
| Catalog Number | HLK 1 |
| Method | LAMP (Loop-mediated Isothermal Amplification) |
| Enzyme | Bst Polymerase (Standard) |
| Reactions | 100 Reactions |
| Sensitivity | 5 molecules of target DNA |
| Incubation Temperature | 60°C to 65°C |
| Compatible Detection | Lateral Flow (MGHD 1), Agarose Gel, Fluorescence |
| Storage | -20°C |
