Product Description
SolisFAST® 1-step RT-PCR Kit with UNG
Description
SolisFAST® 1-step RT-PCR Kit with UNG provides a simple and streamlined workflow for analyzing RNA targets using reverse transcription PCR (RT-PCR). Both the cDNA synthesis and PCR are conveniently performed in one tube greatly simplifying the analysis workflow, reducing reaction-to-reaction variation, minimizing hands-on steps and reducing reaction set-up to results time.
Exceptionally fast and robust SolisFAST® polymerase combined with our highly processive reverse transcriptases make SolisFAST® 1-step RT-PCR Kit with UNG the fastest kit on the market to perform RT-PCR with unparalleled under 1-hour reaction times. Optimized composition ensures superior sensitivity allowing detection of as little as 0.1 pg of RNA and enabling efficient multiplexing of up to 5 RNA targets simultaneously.
Combination of our highly thermostable FIREScript® and SOLIScript® reverse transcriptases allow efficient reactions at temperatures up to 60 °C to facilitate amplifying difficult RNA targets with challenging secondary structures. The RT mix is preblended with our in silico-designed ribonuclease inhibitor RiboGrip® to minimize the risk of RNA degradation. Salini UNG® Uracil-N-Glycosylase is incorporated into the product along with dUTPs to prevent carryover contamination and false positive results.
The kit is supplied with a novel RT-PCR enhancer – AmpliBoostTM to further improve sensitivity and performance with GC-rich and other challenging amplicons.
Applications
SolisFAST® 1-step RT-PCR Kit with UNG is suitable for RT-PCR applications such as:
- Gene expression analysis
- Determining the presence or absence of RNA
- Virus detection
- Cloning of RNA templates up to 2 kb
- Suitable for both singleplex or multiplex (up to 5-targets) RT-PCR reactions
Properties
Sample type: RNA
Product format: reagent kit
Detection type: end-point analysis
Priming method: gene specific primers
Ready-to-load: no
Amplicon Size: up to 2 kb
Targets: 1-5 targets
Reagents
4x One-step HOT SolisFAST® Mix with UNG with HOT SolisFAST® DNA polymerase, Salini UNG® Uracil-N-Glycosylase, optimized reaction buffer, dNTPs including dUTP, 6.4 mM MgCl2 (1x qPCR solution – 1.6 mM MgCl2)
40x One-step RT Mix with SOLIScript® Reverse Transcriptase, FIREScript® Reverse Transcriptase, RiboGrip® RNase Inhibitor, Salini UNG® Uracil-N-Glycosylase.
5x AmpliBoost™ RT-PCR enhancer – A novel RT-PCR enhancer to improve sensitivity and help with challenging templates (e.g. GC-rich, secondary RNA structures).
Water, nuclease free
