Product Description
SolisFAST® 1-step RT-PCR Kit with UNG Ready to Load
Description
SolisFAST® 1-step RT-PCR Kit with UNG provides a simple and streamlined workflow for analyzing RNA targets using reverse transcription PCR (RT-PCR). Both the cDNA synthesis and PCR are conveniently performed in one tube greatly simplifying the analysis workflow, reducing reaction-to-reaction variation, minimizing hands-on steps and reducing reaction set-up to results time.
Exceptionally fast and robust SolisFAST® polymerase combined with our highly processive reverse transcriptases make SolisFAST® 1-step RT-PCR Kit with UNG the fastest kit on the market to perform RT-PCR with unparalleled under 1-hour reaction times. Optimized composition ensures superior sensitivity allowing detection of as little as 0.1 pg of RNA and enabling efficient multiplexing of up to 5 RNA targets simultaneously.
Combination of our highly thermostable FIREScript® and SOLIScript® reverse transcriptases allow efficient reactions at temperatures up to 60 °C to facilitate amplifying difficult RNA targets with challenging secondary structures. The RT mix is preblended with our in silico-designed ribonuclease inhibitor RiboGrip® to minimize the risk of RNA degradation. Salini UNG® Uracil-N-Glycosylase is incorporated into the product along with dUTPs to prevent carryover contamination and false positive results.
The kit is supplied with a novel RT-PCR enhancer – AmpliBoostTM to further improve sensitivity and performance with GC-rich and other challenging amplicons. Ready-to-Load variant comes with inert loading dyes included to directly load the samples on the gel to minimize steps and save time during analysis.
Applications
SolisFAST® 1-step RT-PCR Kit with UNG is suitable for RT-PCR applications such as:
- Gene expression analysis
- Determining the presence or absence of RNA
- Virus detection
- Cloning of RNA templates up to 2 kb
- Suitable for both singleplex or multiplex (up to 5-targets) RT-PCR reactions
Properties
Sample type: RNA
Product format: reagent kit
Detection type: end-point analysis
Priming method: gene specific primers
Ready-to-load: no
Amplicon Size: up to 2 kb
Targets: 1-5 targets
Reagents
4x One-step HOT SolisFAST® Mix with UNG with HOT SolisFAST® DNA polymerase, optimized reaction buffer, dNTPs including dUTP, 6.4 mM MgCl2 (1x qPCR solution – 1.6 mM MgCl2)
40x One-step RT Mix with SOLIScript® Reverse Transcriptase, FIREScript® Reverse Transcriptase, RiboGrip® RNase Inhibitor, Salini UNG® Uracil-N-Glycosylase.
5x AmpliBoost™ RT-PCR enhancer – A novel RT-PCR enhancer to improve sensitivity and help with challenging templates (e.g. GC-rich, secondary RNA structures).
Water, nuclease free
Green loading dye1: Mix of blue and yellow dye to allow tracking of DNA during electrophoresis.
1The mix is not recommended for use in applications where spectrophotometric measurements are necessary (e.g. Sanger sequencing). Migration speed: blue dye – TBE: ~3.5 kb, TAE: ~4.5 kb; yellow dye – TBE: ~35 bp, TAE: ~45 bp).
