Product Description
Highly processive, thermostable Taq DNA polymerase
Genetically modified thermostable Taq DNA Polymerase that provides robust and reproducible results
- robust amplification in routine applications
- suitable for templates up to 5 kb
- reaction set-up and shipment without ice
- TA cloning
Description
FIREPol® is a highly processive, thermostable DNA Polymerase. Due to its genetic modifications, it has enhanced stability at room temperature with no activity loss for up to 1 month.
Recommended for routine applications (fragment up to 3 kb from genomic DNA).
Possesses 5′→3′ polymerase and 5′→3′ exonuclease activity, as well as a non-template-dependent terminal transferase activity, but lacks a 3′→5′ exonuclease (proofreading) activity making the generated product suitable for TA-cloning.
The fidelity of FIREPol® is similar to a regular Taq DNA Polymerase (error rate per nucleotide ca 2.5 x10-5).
Properties
Concentration: 5 U/µl
Error rate per nucleotide per cycle is app. 2.5 x 10-5
Accuracy is app. 4 x 104
Estimated half life at 95ºC is 1.5 hours.
Storage and Dilution Buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH
Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.
Kit Components
FIREPol® DNA Polymerase (5 U/µl) in 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA, 50% glycerol (v/v), and stabilizers.
FIREPol® 10x Buffer B (without Mg2+ ): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4, 0.2% w/v Tween-20.
FIREPol® 10x Buffer B contains non-ionic detergent suppressing inhibitory effects of the traces of DNA extraction buffer and enhancing PCR yield and efficiency.
FIREPol® 10x Buffer BD (without Mg2+ and detergent): 0.8 M Tris-HCl, 0.2 M (NH4)2SO4.
25 mM MgCl2
GC-rich Enhancer is an additive that facilitates the amplification of difficult templates (e.g. GC-rich DNA templates).
Storage
Routine storage: -20ºC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of FIREPol® DNA Polymerase.
Shipping
At room temperature.
| Item | Pcs. | Vial size | |||
| FIREPol® DNA Polymerase | 1 | 100 U | 500 U | 1000 U | 2000 U |
| 10x Reaction Buffer B | 1 | 0.5 ml | 2.5 ml | 5 ml | 5 ml |
| 10x Reaction Buffer BD | 1 | 0.5 ml | 2.5 ml | 5 ml | 5 ml |
| 25 mM MgCl2 | 1 | 0.5 ml | 2.5 ml | 5 ml | 5 ml |
| 10x GC-rich Enhancer | 1 | 0.1 ml | 0.1 ml | 0.5 ml | 0.5 ml |
