Product Description
IBI Cell-Free DNA/RNA Extraction - 100 prep
- Sample: 1ml - 5ml of serum or plasma
- Time: Purify cfDNA/RNA within 60 minutes
- Format: cfDNA/RNA spin columns combined with column extension tubes using centrifuge or vaccuum.
- Yield: 1 - 100ng of cfDNA/RNA per/ml of serum or plasma.
The cfDNA/RNA Extraction kit is designed for rapid isolation of high quality circulating cell-free DNA/RNA from up to 5ml of serum or plasma. The kit includes uniquely designed Column Extension Tubes which allow for increased sample volume and yield. Biological liquid samples are lysed using Proteinase K and a buffer containing chaotropic salt. The lysate is mixed with a binding buffer to facilitate cfDNA/RNA binding to the column membrane. The column is then washed and cfDNA/RNA is eluted with RNAse Free water. The entire procedure can be completed within 60 minutes and the purified cfDNA/RNA is ready for use in a variety of downstream such as qPCR, Next-Generation sequencing and DNA methylation analysis.
Specifications:
- sample size: 1ml - 5ml of serum or plasma
- Time: within 60 minutes
- Format: cfDNA/RNA spin columns combined with column extension tubes using centrifuge or vaccuum.
- Yield: 1 - 100ng of cfDNA/RNA per/ml of serum or plasma
- Elution volume: 30 - 50ul
- Applications: qPCR, Next Generation Sequencing and DNA Metylation Analysis.
- Storage: Dry at room temp. (20 to 25 C)
Protocol
IB47720, IB47721, IB47722, IB47723 Extraction Kit Protocol
