Product Description
CAS# 75-12-7
- Used in hybridization, electrophoresis applications, and to denature DNA and RNA
Spectral Grade Formamide is used as a denaturant in hybridization and is used in hybridization buffers made from standard recipes. Formamide separates the two strands of the double helix. Can also be used as a cryoprotectant and gel stabilizer. Ideal for preparing brain sections of mice for BrdU immunohistochemistry and FISH analysis of HeLa cells.
IBI Formamide is supplied in an amber glass bottle, it is distilled, deionized, and packaged under nitrogen to prevent oxidation. Bottles that have been opened and stored should be deionized prior to use by mixing 1 gm of mixed bed resin per 10 ml of formamide for 30 minutes. It is a clear liquid amide derived from formic acid.
The purity of IBI formamide is determined by way of gas chromatography.
Physical Specifications
CAS#: 75-12-7
Formula Weight: 45.04
Molecular Formula: CH3NO
Purity: Min. 99.0%
Conductivity: Max. 100 μmho
A280: Max. 0.08
Iron: <0.0005%
Copper: <0.00001%
Lead: <0.00005%
Zinc: <0.00005%
Molecular Biology Specifications
DNase assay: None Detected
RNase assay: None Detected
Protease assay: None Detected
